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Deposition of type X collagen in the cartilage extracellular matrix
Author(s) -
Cancedda Ranieri,
Capasso Olga,
Castagnola Patrizio,
DescalziCancedda Fiorella,
Quarto Natalina
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240280103
Subject(s) - extracellular matrix , cartilage , collagen, type i, alpha 1 , type ii collagen , type i collagen , chemistry , matrix (chemical analysis) , embryo , extracellular , messenger rna , cell type , anatomy , microbiology and biotechnology , cell , biochemistry , biology , endocrinology , gene , chromatography
In cultured chick embryo chondrocytes, type X collagen is preferentially deposited in the extracellular matrix, the ratio between type II and type X collagen being about 5 times higher in the culture medium than in the cell layer. When the newly synthesized collagens deposited in slices from the epiphyseal cartilage of 17‐day‐old embryo tibiae were isolated, type X collagen was always the major species. In agreement with this result the mRNA for type X collagen was the predominant mRNA species purified from the same tissue. When the total collagen (unlabeled) deposited in the epiphyseal cartilage was analyzed, it was observed that type X collagen represented only 1/15 of the type II collagen recovered in the same preparation. The possible explanations for these differences are discussed.