Premium
A β‐type transforming growth factor, present in conditioned cell culture medium independent of cell transformation, may derive from serum
Author(s) -
Stromberg Kurt,
Twardzik Daniel R.
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240270412
Subject(s) - transforming growth factor , epidermal growth factor , cell culture , tgf alpha , transformation (genetics) , growth factor , extracellular , clonogenic assay , biology , murine leukemia virus , paracrine signalling , transforming growth factor, beta 3 , transforming growth factor beta , cell type , cell growth , cell , microbiology and biotechnology , chemistry , receptor , biochemistry , genetics , gene
Abstract An alpha‐type transforming growth factor (TGF α ) is produced at high levels by rat embryo cells transformed by the Snyder‐Theilen strain of feline sarcoma virus (FeSV). Addition of 2 ng mouse epidermal growth factor (mEGF) during purification identified the presence of a second, EGF‐dependent growth factor of the TGF beta type (TGF β ) in this conditioned medium. This factor had an approximate Mr of 12,000 and eluted at 37% acetonitrile during high performance liquid chromatography. This extracellular type of TGF β activity also was present in conditioned medium of rat cells after infection with a transformation defective strain of Abelson leukemia virus, and hence expression of this growth factor activity was independent of cell transformation. Moreover, the presence of an EGF‐dependent. 12,000 Mr clonogenic activity in extracts of bovine serum alone suggests scrum as an origin for the B‐type transforming growth factor initially observed in conditioned medium of Snyder‐Theilen FeSV transformed cells. This does not, however, preclude the possibility that TGF β is also secreted by the transformed rat embryo cells themselves.