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Production of a monoclonal antibody directed against the nerve growth factor receptor from sympathetic membranes
Author(s) -
Morgan Claudia J.,
Bradshaw Ralph A.
Publication year - 1985
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240270206
Subject(s) - nerve growth factor , monoclonal antibody , antibody , receptor , clone (java method) , microbiology and biotechnology , chemistry , biology , monoclonal , membrane , endocrinology , medicine , immunology , biochemistry , gene
Spleen cells from BALB/c mice immunized with a plasma membrane‐enriched fraction from rabbit sympathetic ganglia were fused with the mouse myeloma NS1. A hybrid clone was obtained that produced monoclonal antibody directed against the receptor for nerve growth factor (NGF). The antibody, identified as IgG, was able to immunoprecipitate solubilized NGF receptor in the presence or absence of bound NGF. The antibody bound specifically to sympathetic membranes with high affinity but did not affect the binding of 125 I‐NGF to its receptor in sympathetic or sensory neurons or PC12 cells.