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Decreased activity of acetyl‐CoA carboxylase during chemically induced neutrophilic differentiation of human promyelocytic leukemia cells
Author(s) -
Fischkoff Steven A.,
Papuchis Gary C.,
Nickols Wayne A.
Publication year - 1984
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240260203
Subject(s) - retinoic acid , acetyl coa carboxylase , biochemistry , butyric acid , leukemia , pyruvate carboxylase , enzyme , cellular differentiation , fatty acid synthesis , cell culture , fatty acid , chemistry , biosynthesis , enzyme assay , biology , microbiology and biotechnology , immunology , genetics , gene
In order to better understand the mechanism by which changes in the fatty acid composition of cellular lipids occur in leukemia cell lines induced to differentiate, the activity of the first enzyme of fatty acid biosynthesis, acetyl‐CoA carboxylase (EC 6.4.1.2) was measured in HL‐60 promyclocytic leukemia cells before, during and after treatment with compounds that induce these cells to mature to neutro‐phillike cells. After 24 h of exposure to dimethylsulfoxide, retinoic acid, or butyric acid, no morphological or biochemical (nitroblue tetrazolium reduction) evidence of differentiation occurred, but acetyl‐CoA carboxylase activity decreased 44, 44.5, and 49% respectively, compared to untreated cells. After 7 days of culture in the presence of these agents, 79, 83, and 72% of cells acquired the ability to reduce nitroblue tetrazolium (versus 15% of control cells) and enzyme activity decreased 92.7, 99.7, and 98%, compared to control cultures, with the three compounds respectively. Thus, some of the reported changes in fatty acid composition of leukemia cells with differentiation may arise, in part, from the depression of the de novo fatty acid biosynthetic pathway and the loss of acetyl‐CoA carboxylase activity may be a useful marker for neutrophilic differentiation in HL‐60 cells.