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Immunological comparison of desmosomal components from several bovine tissues
Author(s) -
Giudice George J.,
Cohen Stephen M.,
Patel Nipam H.,
Steinberg Malcolm S.
Publication year - 1984
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240260104
Subject(s) - microbiology and biotechnology , biology , chemistry
A panel of monoclonal antibodies and conventional antisera directed against desmosomal proteins from bovine muzzle epidermis was used Io identify immunologically related proteins from two other bovine stratified squamous epithelia, cornea and esophagus. Desmosome‐enriched tissue fractions were prepared from epidermis, cornea, and esophagus. These tissue extracts were electrophoresed on sodium dodecyl sulfate (SDS)‐polyacrylamide gels, blotted onto nitrocellulose paper, and labeled using an indirect immunoperoxidase technique. Labeling with the conventional antisera demonstrates that each of the previously characterized epidermal desmosomal proteins or protein families has an immunologically cross‐reacting counterpart in cornea and esophagus. However, chemical differences between homologous desmosomal proteins in these three tissues have also been detected. The corresponding proteins in the different tissues have similar but not always identical apparent molecular weights. Moreover, tissue‐restricted antigenic determinants were detected in two of the desmosomal protein families using four monoclonal antibodies, each of which recognizes a distinct antigenic determinant.