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Kinetic analyses of the membrane‐bound alkaline phosphatase activity of hymenolepis diminuta (cestoda: Cyclophyllidea) in relation to development of the tapeworm in the definitive host
Author(s) -
Pappas Peter W.
Publication year - 1984
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240250303
Subject(s) - hymenolepis diminuta , alkaline phosphatase , cestoda , 5' nucleotidase , membrane , biology , cestode infections , biochemistry , chemistry , biophysics , helminths , immunology , enzyme
The specific activities of the alkaline phosphatase (APase), type I phosphodiesterase and 5′‐nucleotidase activities associated with the brush‐border plasma membrane of the tapeworm, Hymenolepis diminuta, decrease significantly as the tapeworm grows and matures. Kinetic analyses of the APase activity associated with membrane preparations from whole 6‐, 12‐, and 18‐d‐old H diminuta, and individual pieces of 18‐d‐old H diminuta cut into ten pieces of equal length, failed to demonstrate qualitative changes in the APase activity. Therefore, the decreased specific activities are apparently due to changes in the ratios of enzymatically active to enzymatically inactive membrane proteins (ie, quantitative changes in the membrane proteins) which occur as the tapeworm grows.