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Role of membrane thermotropic properties on hypotonic hemolysis and hypertonic cryohemolysis of human red blood cells
Author(s) -
Minetti Maurizio,
Ceccarini Marina,
Maria Anna,
Di Stasi M.
Publication year - 1984
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240250202
Subject(s) - thermotropic crystal , hemolysis , tonicity , echinocyte , red blood cell , chemistry , membrane , lysis , glutaraldehyde , biophysics , erythrocyte fragility , biochemistry , chromatography , biology , organic chemistry , immunology , liquid crystalline , phase (matter)
The hypothesis of a correlation between the effects of temperature on red blood cells hypotonic hemolysis and hypertonic cryoheniolysis and two thermotropic structural transitions evidenced by EPR studies has been tested. Hypertonic cryoheniolysis of red blood cells shows critical temperatures at 7°C and 19°C. In hypotonic solution, the osmotic resistance increases near 10°C and levels off above 20°C. EPR studies of red blood cell membrane of a 16‐dinyloxyl stearic acid spin label show, in the 0–50°C range, the presence of three thermotropic transitions at 8, 20, and 40°C. Treatments of red blood cells with acidic or alkaline pH, glutaraldehyde, and chlorpromazine abolish hypertonic cryoheniolysis and reduce the effect of temperature on hypotonic hemolysis. 16‐Dinyloxyl stearic acid spectra of red blood cells treated with glutaraldehyde and chlorpromazine show the disappearance of the 8°C transition. Both the 8°C and the 20°C transitions were abolished by acidic pH treatment. The correlation between the temperature dependence of red blood cell lysis and thermotropic breaks might be indicative of the presence of structural transitions producing areas of mismatching between differently ordered membrane components where the osmotic resistance is decreased.