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Hyperthermia‐enhanced TRAIL‐ and mapatumumab‐induced apoptotic death is mediated through mitochondria in human colon cancer cells
Author(s) -
Song Xinxin,
Kim HanCheon,
Kim SeogYoung,
Basse Per,
Park BaeHang,
Lee ByeongChel,
Lee Yong J.
Publication year - 2012
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24023
Subject(s) - apoptosis , cytochrome c , colorectal cancer , hyperthermia , programmed cell death , caspase , cancer research , hepatocyte , mitochondrion , cytotoxicity , cytosol , reactive oxygen species , cancer cell , receptor , fas ligand , poly adp ribose polymerase , chemistry , biology , medicine , cancer , microbiology and biotechnology , biochemistry , polymerase , in vitro , enzyme
Colorectal cancer is the third leading cause of cancer‐related mortality in the world; death usually results from uncontrolled metastatic disease. Previously, we developed a novel strategy of TNF‐related apoptosis‐inducing ligand (Apo2L/TRAIL) in combination with hyperthermia to treat hepatic colorectal metastases. However, previous studies suggest a potential hepatocyte cytotoxicity with TRAIL. Unlike TRAIL, anti‐human TRAIL receptor antibody induces apoptosis without hepatocyte toxicity. In this study, we evaluated the anti‐tumor efficacy of humanized anti‐death receptor 4 (DR4) antibody mapatumumab (Mapa) by comparing it with TRAIL in combination with hyperthermia. TRAIL, which binds to both DR4 and death receptor 5 (DR5), was approximately tenfold more effective than Mapa in inducing apoptosis. However, hyperthermia enhances apoptosis induced by either agent. We observed that the synergistic effect was mediated through elevation of reactive oxygen species, c‐Jun N‐terminal kinase activation, Bax oligomerization, and translocalization to the mitochondria, loss of mitochondrial membrane potential, release of cytochrome c to cytosol, activation of caspases, and increase in poly(ADP‐ribose) polymerase cleavage. We believe that the successful outcome of this study will support the application of Mapa in combination with hyperthermia to colorectal hepatic metastases. J. Cell. Biochem. 113: 1547–1558, 2012. © 2011 Wiley Periodicals, Inc.