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Nuclear protein changes following N,N‐dimethylformamide (DMF)‐induced maturation
Author(s) -
Kimball P. M.,
Hixon Sharon
Publication year - 1983
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240220406
Subject(s) - sodium dodecyl sulfate , dimethylformamide , gel electrophoresis , chemistry , metabolism , cell culture , microbiology and biotechnology , nuclear protein , sodium , electrophoresis , cell , leucine , biochemistry , chromatography , gene , biology , amino acid , solvent , transcription factor , genetics , organic chemistry
A human colonic carcinoma cell line was exposed to a nontoxic concentration of N,N‐dimethylformamide (DMF) for 2 wk. Nuclear proteins were isolated from control and treated cells and compared by sodium dodecyl sulfate (SDS) electrophoresis. Qualitative and quantitative differences were observed. Metabolic labeling with tritiated leucine demonstrated qualitative variation between control and treated cells.