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A simian virus 40‐encoded protein of M r 74,000 daltons is structurally related to the capsid proteins of the virus
Author(s) -
Krippl Bernd,
Dreiseikelmann Brigitte,
Werchau Hermann
Publication year - 1983
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240220402
Subject(s) - capsid , lytic cycle , virus , methionine , biology , microbiology and biotechnology , amino acid , virology , biochemistry , chemistry
We have demonstrated the synthesis of a 74,000‐dalton protein (74K protein) in African green monkey kidney cells infected with simian virus (SV)40. The 74K protein was detected late during the lytic cycle. Its synthesis was inhibited by arabinosyl cytosine as was the synthesis of the capsid proteins. Monospecific antibodies raised against VP1 and VP3 precipitated the structural proteins and the 74K protein. The 74K protein was not found in purified virions. Tryptic peptide analysis demonstrated that the 74K protein shares methionine‐ and serine‐containing peptides with VP1 and VP3 and thus is structurally related to the capsid proteins.