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Core substructure in cyanobacterial phycobilisomes
Author(s) -
Gingrich Jeffrey C.,
Lundell Daniel J.,
Glazer Alexander N.
Publication year - 1983
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240220102
Subject(s) - phycobilisome , allophycocyanin , phycocyanobilin , thylakoid , cyanobacteria , synechococcus , protein subunit , chemistry , phycobiliprotein , crystallography , biophysics , biology , phycocyanin , microbiology and biotechnology , biochemistry , chloroplast , genetics , gene , bacteria
The tricylindrical core of Synechocystis 6701 phycobilisomes is made up of four types of allophycocyanin‐containing complexes: A, (α AP β AP ) 3 ; B, (α AP β AP ) 3 .10K; C, (α 1 APB α 2 AP β 3 AP ).10K; D, (α AP β AP ) 2 .18.5K.99K; where AP is allophycocyanin, APB is allophycocyanin B, and 10K, 18.5K, and 99K are polypeptides of 10,000, 18,500, and 99,000 daltons, respectively. The 18.5K polypeptide is a hitherto unrecognized biliprotein subunit with a single phycocyanobilin prosthetic group. The tricylindrical core is made up of 12 subcomplexes in the molar ratio of A:B:C:D: of 4:4:2:2. Complexes C and D act as terminal energy acceptors. From these results and previous analysis of the bicylindrical core of Synechococcus 6301 phycobilisomes [14,15] it is proposed that the two cylinders of the Synechocystis 6701 core, proximal to the thylakoid membrane, each have the composition ABCD, and that the distal cylinder has the composition A 2 B 2 .