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Immunological studies of β‐adrenergic receptors
Author(s) -
Couraud P. O.,
Lu B. Z.,
Schmutz A.,
DurieuTrautmann O.,
KlutchkoDelavier C.,
Hoebeke J.,
Strosberg A. D.
Publication year - 1983
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240210301
Subject(s) - polyclonal antibodies , receptor , monoclonal antibody , antibody , immunoprecipitation , adrenergic receptor , microbiology and biotechnology , chemistry , biology , biochemistry , immunology
Two types for antibodies have been raised against the β‐adrenergic receptor: either by injection of highly purified receptor from turkey erythrocytes or by injection of anticatecholamine ligand antibodies, and induction of anti‐idiotypic antibodies Our data illustrate the interactions of the β‐adrenergic receptor with these polyclonal antibodies. Preliminary results with monoclonal antibodies are also described. The redistribution of β‐receptors on intact cells is visualized by the use of fluorescent antibodies. Immunoprecipitation of radioiodinated receptor by the antireceptor antibodies yields a single major 60,000 MW component.