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Estrogen receptor‐mediated cytotoxicity using iodine‐125
Author(s) -
Bloomer W. D.,
McLaughlin W. H.,
Milius R. A.,
Weichselbaum R. R.,
Adelstein S. J.
Publication year - 1983
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240210106
Subject(s) - cytotoxicity , antiestrogen , estrogen receptor , dna , chemistry , nucleus , steroid , cytoplasm , cancer research , receptor , estrogen , microbiology and biotechnology , biology , hormone , medicine , biochemistry , endocrinology , breast cancer , cancer , in vitro , genetics
Auger effects from 125 I decay are singularly damaging if localized in DNA as the thymidine analogue 125 I‐iododeoxyuridine ( 125 IUdR). Recent experience with steroid sex hormones extends these observations by demonstrating cytotoxicity in sites other than the DNA backbone. We have compared the cytotoxicity in human MCF‐7 breast cancer cells of 125 IUdR, 125 I‐iodotamoxifen, a nonsteroidal antiestrogen that is translocated from the cytoplasm to the nucleus of receptor containing cells, and 125 I‐iodoantipyrine, a biological indicator of the body water space. Cytotoxicity is critically dependent upon subcellular localization.