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Monoclonal antibodies to feline sarcoma virus gag and fes gene translational products
Author(s) -
Veronese Fulvia,
Kelloff Gary J.,
Reynolds Fred H.,
Hill Richard W.,
Stephenson John R.
Publication year - 1983
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240210103
Subject(s) - polyproteins , antibody , monoclonal antibody , group specific antigen , virology , biology , gene , neurotropic virus , microbiology and biotechnology , virus , genetics , genome
A series of hybridomas have been isolated which produce monoclonal antibodies directed against polyprotein gene products of the Gardner, Snyder‐Theilen, and McDonough strains of FeSV. Within these are representatives of several immunoglobulin classes including IgG 1 , IgG 2a , IgG 2b , IgG 2c , and IgM. Antibody produced by one hybridoma recognizes immunologic determinants localized within an FeLV gag gene structural component (p15) common to polyproteins encoded by all three FeSV isolates whereas antibody produced by a second is specific for p30 determinants unique to P170 gag‐fms . Additional hybridomas secrete antibody directed against v‐ fes ‐encoded determinants common to the Gardner and Snyder‐Theilen FeSV‐encoded polyproteins. GA P110 gag‐fes and ST P85 gag‐fes immunoprecipitated by antibody directed against p15 exhibit tyrosine‐specific protein kinase activity but lack such activity when precipitated by antibody specific for their acquired sequence (v‐ fes ) components.