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Organization and expression of hepatitis B sequences cloned from hepatocellular carcinoma tissue DNA
Author(s) -
Dejean Anne,
Carloni Guido,
Bréchot Christian,
Tiollais Pierre,
WainHobson Simon
Publication year - 1982
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240200309
Subject(s) - hepatitis b virus , hbsag , biology , virology , clone (java method) , microbiology and biotechnology , hepatocellular carcinoma , dna , gene , genomic library , virus , cancer research , genetics , peptide sequence
We have constructed a phage λ library of liver DNA fragments from West African patient who died of liver failure due to advanced hepatocellular carcinoma. Four hepatitis B virus (HBV) DNA‐carrying recombinants have been isolated, one clone (λÍ22) being analyzed in greatest detail. It contains approximately 3.8 kb of HBV DNA without detectable deletions or rearrangements. One site of integration lies close to the nick in free viral DNA. The restriction map of the HBV sequences is close to those published for the ay subtype. Coconvection of mouse Ltk − cells with λÍ22 and cloned: thymidine kinase gene results in the expression of gene S and the excretion of hepatitis B surface antigen (HBsAg) particles into the culture supernatant.