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Binding of isolated 3T3 surface membranes to growing 3T3 cells and their effect on cell growth
Author(s) -
Lieberman Michael A.,
KellerMcGandy Christine E.,
Woolsey Thomas A.,
Glaser Luis
Publication year - 1982
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240200109
Subject(s) - membrane , 3t3 cells , cell , cell growth , cell membrane , population , biophysics , microbiology and biotechnology , cell cycle , biology , chemistry , biochemistry , cell culture , transfection , genetics , demography , sociology
We have quantitated by autoradiography the binding of [ 125 I]labeled 3T3 plasma membrane fragments to 3T3 cells growing on the surface of plastic dishes; ie, the same conditions in which these membranes specifically arrest the growth of 3T3 cells early in the G 1 phase of the cell cycle. We have been able to demonstrate that binding of membranes to cells is coincidental with the expression of the growth inhibitory activity of protein(s) present in the membrane fragments. Treatments that reduce binding (heat denaturation of the membranes or culture in the presence of high scrum) also reduce growth inhibitory activity. [ 125 I]labeled membranes bound to cells are located primarily on the cell surface (as determined by electron microscope autoradiography) and are exchangeable with unlabeled membranes. We conclude that binding of membranes to cells is necessary but may not be sufficient for the expression of the growth inhibitory activity of these membranes. This approach provides information not only on the average level of binding of membranes to cells, but also provides a quantitative assessment of the variation of the level of membrane to cell binding between different cells in the population.