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Multiple specific binding sites for purified glucocorticoid receptors on mammary tumor virus DNA
Author(s) -
Payvar Farhang,
Firestone Gary L.,
Ross Susan R.,
Chandler Vicki L.,
Wrange Örjan,
CarlstedtDuke Jan,
Gustafsson Janåke,
Yamamoto Keith R.
Publication year - 1982
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240190305
Subject(s) - mouse mammary tumor virus , glucocorticoid receptor , biology , microbiology and biotechnology , transcription (linguistics) , dna , promoter , transcription factor , binding site , in vitro , virus , receptor , gene , virology , biochemistry , gene expression , linguistics , philosophy
Abstract Glucocorticoid hormones selectively stimulate the rate of transcription of integrated mammary tumor virus (MTV) sequences in infected rat hepatoma cells. Using two independent assays, we find that purified rat liver glucocorticoid receptor protein binds specifically to at least four widely separated regions on pure MTV proviral DNA. One of these specific binding domains, which itself contains at least two distinct receptor binding sites, resides within a fragment of viral DNA that maps 110–449 bp upstream of the promoter for MTV RNA synthesis. Three other binding domains lie downstream of the promoter and within the MTV primary transcription unit. Restriction fragments bearing separate binding domains have been introduced into cultured cells; transformants have been recovered in which the introduced fragments arc expressed under glucocorticoid control. Thus, it appears that this assay will be useful for assessing the biological significance of the receptor binding sites detected in vitro.