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Hypoxia inhibits the spontaneous calcification of bone marrow‐derived mesenchymal stem cells
Author(s) -
Huang YongCan,
Zhu HongMing,
Cai JiaQin,
Huang YiZhou,
Xu Jin,
Zhou Yi,
Chen XiaoHe,
Li XiuQun,
Yang ZhiMing,
Deng Li
Publication year - 2012
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24014
Subject(s) - mesenchymal stem cell , osteocalcin , calcification , alkaline phosphatase , runx2 , hypoxia (environmental) , bone marrow , chemistry , calcium , microbiology and biotechnology , immunology , pathology , andrology , biology , medicine , biochemistry , enzyme , oxygen , organic chemistry
Bone marrow‐derived mesenchymal stem cells (BM‐MSCs) are the popular seed cells for regenerative medicine, and there has been a rapid increase in the number of BM‐MSC‐based clinical trials. However, the safety of these cells should also be closely studied. In this study, spontaneous calcification of BM‐MSCs from rats was evaluated in normoxia (20% O 2 ) without osteogenic medium after continuous culture for 21 days; obvious mineralized nodules were observed, which were positive for Alizarin Red, collagen‐I (Col‐I), osteocalcin (OC) and alkaline phosphatase (ALP), and mainly consisted of C, O and Ca elements. Interestingly, hypoxia (2% O 2 ) significantly inhibited this spontaneous calcification. In addition, the ALP and calcium content of rBM‐MSCs were sharply reduced. Based on RT‐PCR results, the expression of osteogenic genes (Cbfa1/Runx2, Col‐I, ALP, and OC) was reduced compared to that in normoxia. These results demonstrate a natural and unique characterization of rat BM‐MSCs in normoxia after continuous culture and highlight the inhibiting effects of hypoxia. Finally, this study contributes to the information regarding the application of BM‐MSCs in the regeneration of various tissues. J. Cell. Biochem. 113: 1407–1415, 2012. © 2011 Wiley Periodicals, Inc.