Premium
Upregulation of recepteur d'origine nantais tyrosine kinase and cell invasiveness via early growth response‐1 in gastric cancer cells
Author(s) -
Lee Ko E.,
Park Jung S.,
Khoi Pham N.,
Joo Young E.,
Lee Young H.,
Jung Young D.
Publication year - 2012
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.23454
Subject(s) - downregulation and upregulation , cancer research , microbiology and biotechnology , carcinogenesis , biology , cell growth , cell , phorbol ester , phorbol , cancer , chemistry , signal transduction , protein kinase c , gene , biochemistry , genetics
Abnormal accumulation and activation of the recepteur d'origine nantais (RON) has been implicated in carcinogenesis of epithelial tumors. RON expression was induced by the tumor promoter, phorbol 12‐myristate 13‐acetate (PMA), in gastric adenocarcinoma AGS cells. Studies with deleted and site‐directed mutagenesis of Egr‐1 promoter and with expression vectors encoding Egr‐1 confirmed that Egr‐1 is essential for RON expression. In addition, AGS cells pretreated with PMA showed remarkably enhanced invasiveness, which was partially abrogated by siRNA‐targeted RON and Egr‐1. These results suggest that tumor promoter induces RON expression via Egr‐1, which, in turn, stimulates cell invasiveness in AGS cells. J. Cell. Biochem. 113: 1217–1223, 2012. © 2011 Wiley Periodicals, Inc.