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The role of kupffer cells in rat liver regeneration revealed by cell‐specific microarray analysis
Author(s) -
Xu CunShuan,
Jiang Yun,
Zhang LianXing,
Chang CuiFang,
Wang GaiPing,
Shi RuiJia,
Yang YanJie
Publication year - 2012
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.23348
Subject(s) - liver regeneration , regeneration (biology) , biology , microbiology and biotechnology , microarray analysis techniques , kupffer cell , cell , gene expression , microarray , gene , immunology , genetics
Liver regeneration after partial hepatectomy is a process with various types of cells involved. The role of Kupffer cells (KCs) in liver regeneration is still controversial. In this study we isolated KCs from regenerating liver and conducted cell‐specific microarray analysis. The results demonstrated that the controversial role of KCs in liver regeneration could be explained with the expression patterns of TGF‐α, IL‐6, TNF, and possibly IL‐18 during liver regeneration. IL‐18 may play an important role in negative regulation of liver regeneration. The functional profiles of gene expression in KCs also indicated that KC signaling might play a negative role in cell proliferation: signaling genes were down regulated before cell division. Immune response genes in KCs were also down regulated during liver regeneration, demonstrating similar expression profiles to that of hepatocytes. The expression patterns of key genes in these functional categories were consistent with the temporal functional profiles. J. Cell. Biochem. 113: 229–237, 2012. © 2011 Wiley Periodicals, Inc.