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Recombinant BMP 4/7 fusion protein induces differentiation of bone marrow stem cells
Author(s) -
Yuan Shaohui,
Pan Qi,
Liu Wei,
Wu Binqi,
Han Xiguang,
Bi Zhenggang
Publication year - 2011
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.23230
Subject(s) - bone morphogenetic protein 6 , bone morphogenetic protein 4 , bone morphogenetic protein , bone morphogenetic protein 7 , osteocalcin , alkaline phosphatase , fusion protein , stem cell , bone marrow , microbiology and biotechnology , bone morphogenetic protein 2 , chemistry , mesenchymal stem cell , recombinant dna , biology , immunology , biochemistry , in vitro , enzyme , gene
Bone morphogenetic proteins (BMPs) induce differentiation of mesenchymal cells to cartilage and bone. We cloned BMP4 and BMP7 cDNAs from human placenta and fetal cartilage cells, respectively, and used an Escherichia coli expression system to produce recombinant BMP4 and BMP4/7 proteins. Differentiation of primary cultures of bone marrow stem cells (BMSC) treated with BMP4 or BMP4/7 was evaluated by Von Kossa staining and by determining alkaline phosphatase activity and osteocalcin level. BMP4/7‐induced BMSC differentiation more potently than BMP4. We showed that BMP4/7 fusion protein expressed in E. coli is biologically active and is a novel strategy to treat bone injury in a clinical setting. J. Cell. Biochem. 112: 3054–3060, 2011. © 2011 Wiley‐Liss, Inc.