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Differential expression of complement proteins in cerebrospinal fluid from active multiple sclerosis patients
Author(s) -
Li Yun,
Qin Zhaoyu,
Yang Mingchong,
Qin Yanjiang,
Lin Chengzhao,
Liu Shilian
Publication year - 2011
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.23113
Subject(s) - multiple sclerosis , complement system , pathogenesis , cerebrospinal fluid , demyelinating disease , blot , complement (music) , immunology , central nervous system , differential diagnosis , biology , pathology , medicine , chemistry , gene , antibody , neuroscience , biochemistry , complementation , phenotype
Multiple sclerosis (MS) is a demyelinating disease of the central nervous system with complex immunopathogenesis. Using the 2‐D DIGE technology, we separate CSF proteins from patients with active MS and control subjects. Three of the seven differential proteins identified were related with complement system, and the network analysis of the differential proteins revealed complement activation involvement in active MS. Complement C4b (gamma chain) was confirmed elevated by performing western blotting analysis ( P < 0.01). The present results are an independent quantitative proteomic measure in CSF from active MS patients. The differential expression of the complement C4b and related proteins in CSF provides potential biomarkers as well as evidence for the involvement of complement activation in the pathogenesis of MS disease. J. Cell. Biochem. 112: 1930–1937, 2011. © 2011 Wiley‐Liss, Inc.