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The localization of hnRNP A2/B1 in nuclear matrix and the aberrant expression during the RA‐induced differentiation of human neuroblastoma SK‐N‐SH cells
Author(s) -
Liang Ying,
Shi SongLin,
Li QiFu,
Chen LanYing,
Jing GuangJun,
Tan GuoWei,
Wang SanYing,
Wu FuYun
Publication year - 2011
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.23063
Subject(s) - nuclear matrix , heterogeneous nuclear ribonucleoprotein , microbiology and biotechnology , ribonucleoprotein , cell nucleus , heterogeneous ribonucleoprotein particle , nuclear protein , gene expression , chemistry , biology , rna , nucleus , gene , transcription factor , biochemistry , chromatin
Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is involved in the synthesis of RNA. Its expression is up‐regulated in many tumor cell lines. In this study, we investigated the distribution of hnRNP A2/B1 in the nuclear matrix, including its co‐localization with expression products of related genes. Results from 2‐DE PAGE and MS showed that hnRNP A2/B1 is involved with components of nuclear matrix proteins of SK‐N‐SH cells, and that its expression level is down‐regulated after retinoic acid (RA) treatment. Protein immunoblotting results further confirm the existence of hnRNP A2/B1 in the nuclear matrix, as well as its down‐regulation after RA treatment. Immunofluorescence microscopy observation showed that hnRNP A2/B1 localized in nuclear matrix of SK‐N‐SH cells and its distribution regions were altered after RA treatment. Laser scanning confocal microscopy observation showed that hnRNP A2/B1 co‐localized with c‐Myc, c‐Fos, P53, and Rb in SK‐N‐SH cells. The co‐localized region was altered as a result of RA treatment. Our data proved that hnRNP A2/B1 is a nuclear matrix protein and can be up‐regulated in human neuroblastoma. The expression and distribution of hnRNP A2/B1 can affect the differentiation of SK‐N‐SH cells, as well as its co‐localization with related oncogenes and tumor suppressor genes. J. Cell. Biochem. 112: 1722–1729, 2011. © 2011 Wiley‐Liss, Inc.