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Inhibition of thyroid‐restricted genes by follicular thyroglobulin involves iodinated degree
Author(s) -
Huang Huibin,
Shi Yaxiong,
Lin Ling,
Li Liangyi,
Lin Xiahong,
Li Xisheng,
Xu Dongming
Publication year - 2011
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.23014
Subject(s) - thyroglobulin , thyroid , follicular phase , organification , medicine , endocrinology , chemistry , iodine , follicular cell , thyroid peroxidase , hormone , biology , organic chemistry
Follicular thyroglobulin (TG) reflects the storage of both iodine and thyroid hormone. This is because it is a macromolecular precursor of thyroid hormone and organic iodinated compound in follicular lumen. Thus, it may have an important feedback role in thyroid function. In this study, monolayer cells were cultured and follicles were reconstituted with primary pig thyroid cells in vitro. Reconstituted follicles were treated with iodine and methimazole (MMI), a drug that blocks iodine organification and reduces the degree of TG iodination in follicular lumen. The high degree of iodinated TG in follicular lumen was observed to inhibit thyroid‐restricted gene expression. To confirm this finding, monolayer thyroid cells were treated with a different degree of TG iodination at the same concentration. These iodinated TG were extracted from reconstituted follicles of different groups. In this manner, this study provides firsthand evidence suggesting that follicular TG inhibits the expressions of thyroid‐restricted genes NIS, TPO, TG, and TSHr. J. Cell. Biochem. 112: 971–977, 2011. © 2011 Wiley‐Liss, Inc.