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Bovine ( Bos taurus ) humoral immune response against Echinococcus granulosus and hydatid cyst infertility
Author(s) -
Paredes Rodolfo,
Godoy Pablo,
Rodríguez Betsabé,
García María Pía,
Cabezón Carolina,
Cabrera Gonzalo,
Jiménez Verónica,
Hellman Ulf,
Sáenz Leonardo,
Ferreira Arturo,
Galanti Norbel
Publication year - 2011
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22916
Subject(s) - echinococcus granulosus , biology , infertility , antigen , immune system , antibody , parasite hosting , immunology , cyst , hydatid cyst , pathology , pregnancy , medicine , zoology , genetics , world wide web , computer science
Echinococcus granulosus , the agent of hydatid disease, presents an indirect life cycle, with canines (mainly dogs) as definitive hosts, and herbivores and human as intermediary ones. In intermediary hosts fertile and infertile cysts develop, but only the first ones develop protoscoleces, the parasite form infective to definitive hosts. We report the presence of bovine IgGs in the germinal layer from infertile cysts (GLIC), in an order of magnitude greater than in the germinal layer from fertile cysts (GLFC). When extracted with salt solutions, bovine IgGs from GLIC are associated with low or with high affinity (most likely corresponding to non specific and antigen specific antibodies, respectively). Specific IgGs penetrate both the cells of the germinal layer and HeLa cultured cells and recognize parasitic proteins. These results, taken together with previous ones from our laboratory, showing induction of apoptosis in the germinal layer of infertile hydatid cysts, provide the first coherent explanation of the infertility process. They also offer the possibility of identifying the parasite antigens recognized, as possible targets for immune modulation. J. Cell. Biochem. 112: 189–199, 2011. © 2010 Wiley‐Liss, Inc.