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Anti‐inflammatory activity of Eugenia punicifolia extract on muscular lesion of mdx dystrophic mice
Author(s) -
Leite Paulo Emílio Corrêa,
de Almeida Kessiane Belshoff,
LagrotaCandido Jussara,
Trindade Pablo,
da Silva Rafael Ferreira,
Ribeiro Manuel Gustavo L.,
LimaAraújo Katia G.,
Santos Wilson C.,
QuiricoSantos Thereza
Publication year - 2010
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22906
Subject(s) - myogenin , muscular dystrophy , inflammation , fibrosis , lesion , chemistry , tumor necrosis factor alpha , endocrinology , proinflammatory cytokine , duchenne muscular dystrophy , skeletal muscle , medicine , in vivo , acetylcholinesterase , mdx mouse , dystrophin , biology , pathology , biochemistry , enzyme , myogenesis , microbiology and biotechnology
Eugenia punicifolia known as “pedra‐ume caá” is a shrub largely distributed in the Amazon region popularly used in decoctions or infusions as a natural therapeutic agent, which can interfere on cholinergic nicotinic neurotransmission. This work aimed to investigate a putative anti‐inflammatory effect of dichloromethane fraction of E. punicifolia extract ( Ep ‐CM) in the muscular lesion of mdx dystrophic mice, considering that activation of cholinergic mechanisms mitigates inflammation. A polymer containing the Ep ‐CM was implanted in mdx gastrocnemius muscle before onset of myonecrosis for local slow and gradual release of bioactive compounds and mice sacrificed 7 days or 9 weeks after surgery. Comparing to control muscle, treatment did not alter choline acetyltransferase and acetylcholinesterase enzymatic activities, but decreased metaloproteases‐9 and ‐2 activities and levels of tumor necrosis factor α and NFκB transcription factor. In addition, treatment also reduced levels of bioactive IL‐1β form and cleaved caspase‐3, related to early events of cellular death and inflammatory activation and further increased myogenin expression without affecting collagen production which is associated with fibrosis. In vivo treatment of mdx dystrophic mice with Ep ‐CM caused significant reduction of muscular inflammation and improved skeletal muscle regeneration without inducing fibrosis. J. Cell. Biochem. 111: 1652–1660, 2010. © 2010 Wiley‐Liss, Inc.