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Differentiation of cardiomyocytes from human embryonic stem cells is accompanied by changes in the extracellular matrix production of versican and hyaluronan
Author(s) -
Chan Christina K.,
Rolle Marsha W.,
PotterPerigo Susan,
Braun Kathleen R.,
Van Biber Benjamin P.,
Laflamme Michael A.,
Murry Charles E.,
Wight Thomas N.
Publication year - 2010
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22744
Subject(s) - versican , extracellular matrix , proteoglycan , microbiology and biotechnology , chondroitin sulfate proteoglycan , embryonic stem cell , chondroitin sulfate , chemistry , cellular differentiation , glycosaminoglycan , aggrecan , biochemistry , biology , pathology , gene , medicine , articular cartilage , alternative medicine , osteoarthritis
Abstract Proteoglycans and hyaluronan play critical roles in heart development. In this study, human embryonic stem cells (hESC) were used as a model to quantify the synthesis of proteoglycans and hyaluronan in hESC in the early stages of differentiation, and after directed differentiation into cardiomyocytes. We demonstrated that both hESC and cardiomyocyte cultures synthesize an extracellular matrix (ECM) enriched in proteoglycans and hyaluronan. During cardiomyocyte differentiation, total proteoglycan and hyaluronan decreased and the proportion of proteoglycans bearing heparan sulfate chains was reduced. Versican, a chondroitin sulfate proteoglycan, accumulated in hESC and cardiomyocyte cultures. Furthermore, versican synthesized by hESC contained more N‐ and O‐linked oligosaccharide than versican from cardiomyocytes. Transcripts for the versican variants, V0, V1, V2, and V3, increased in cardiomyocytes compared to hESC, with V1 most abundant. Hyaluronan in hESC had lower molecular weight than hyaluronan from cardiomyocyte cultures. These changes were accompanied by an increase in HAS‐1 and HAS‐2 mRNA in cardiomyocyte cultures, with HAS‐2 most abundant. Interestingly, HAS‐3 was absent from the cardiomyocyte cultures, but expressed by hESC. These results indicate that human cardiomyocyte differentiation is accompanied by specific changes in the expression and accumulation of ECM components and suggest a role for versican and hyaluronan in this process. J. Cell. Biochem. 111: 585–596, 2010. © 2010 Wiley‐Liss, Inc.