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OCT3/4 regulates transcription of histone deacetylase 4 ( Hdac4 ) in mouse embryonic stem cells
Author(s) -
Addis Russell C.,
Prasad Megana K.,
Yochem Robert L.,
Zhan Xiangcan,
Sheets Timothy P.,
Axelman Joyce,
Patterson Ethan S.,
Shamblott Michael J.
Publication year - 2010
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22707
Subject(s) - hdac4 , histone deacetylase 2 , hdac1 , homeobox protein nanog , sox2 , histone deacetylase , chromatin immunoprecipitation , histone deacetylase 5 , biology , microbiology and biotechnology , embryonic stem cell , chromatin , transcription factor , chemistry , histone , induced pluripotent stem cell , genetics , gene , promoter , gene expression
OCT3/4 is a POU domain transcription factor that is critical for maintenance of pluripotency and self‐renewal by embryonic stem (ES) cells and cells of the early mammalian embryo. It has been demonstrated to bind and regulate a number of genes, often in conjunction with the transcription factors SOX2 and NANOG. In an effort to further understand this regulatory network, chromatin immunoprecipitation was used to prepare a library of DNA segments specifically bound by OCT3/4 in undifferentiated mouse ES (mES) cell chromatin. One segment corresponds to a region within the first intron of the gene encoding histone deacetylase 4 ( Hdac4 ), a Class II histone deacetylase. This region acts as a transcriptional repressor and contains at least two functional sites that are specifically bound by OCT3/4. HDAC4 is not expressed in the nuclei of OCT3/4+ mES cells and is upregulated upon differentiation. These findings demonstrate the participation of OCT3/4 in the repression of Hdac4 in ES cells. J. Cell. Biochem. 111: 391–401, 2010. © 2010 Wiley‐Liss, Inc.