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Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase‐4 (TIMP‐4) in formalin‐fixed, paraffin‐embedded human tissues
Author(s) -
Donover P. Scott,
Wojciechowski Brian S.,
Thirumaran Rajesh,
ZembaPalko Vlasta,
Prendergast George C.,
Wallon U. Margaretha
Publication year - 2010
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22641
Subject(s) - monoclonal antibody , immunohistochemistry , clone (java method) , antibody , tissue microarray , microbiology and biotechnology , metalloproteinase , biology , tissue inhibitor of metalloproteinase , monoclonal , recombinant dna , matrix metalloproteinase , pathology , immunology , medicine , biochemistry , gene , dna
Abstract Overexpression of the extracellular metalloproteinase inhibitor TIMP‐4 in estrogen receptor‐negative breast cancers was found recently to be associated with a poor prognosis for survival. To pursue exploration of the theranostic applications of TIMP‐4, specific antibodies with favorable properties for immunohistochemical use and other clinical assays are needed. Here we report the characterization of a monoclonal antibody (clone 9:4–7) specific for full‐length human TIMP‐4 with suitable qualities. The antibody was determined to be an IgG 2b immunoglobulin. In enzyme‐linked immunosorbent assay (ELISA) and immunoblotting assays, it did not exhibit any detectable crossreactivity with recombinant forms of the other human TIMPs 1, 2, and 3. In contrast, the antibody displayed high specificity and sensitivity for TIMP‐4 including in formalin‐fixed and paraffin‐embedded specimens of human breast specimens. An analysis of tissue microarrays of human cancer and corresponding normal tissues revealed specific staining patterns with excellent signal‐to‐noise ratios. This study documents TIMP‐4 monoclonal antibody clone 9:4–7 as an effective tool for preclinical and clinical investigations. J. Cell. Biochem. 110: 1255–1261, 2010. Published 2010 Wiley‐Liss, Inc.