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Expression of a novel tropomyosin isoform in axolotl heart and skeletal muscle
Author(s) -
Thomas Anish,
Rajan Sudarsan,
Thurston Harold L.,
Masineni Sreeharsha N.,
Dube Preeti,
Bose Abhishek,
Muthu Vasundhara,
Dube Syamalima,
Wieczorek David F.,
Poiesz Bernard J.,
Dube Dipak K.
Publication year - 2010
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22599
Subject(s) - axolotl , tropomyosin , skeletal muscle , myofibril , gene isoform , myocyte , biology , sarcomere , cardiac muscle , regeneration (biology) , microbiology and biotechnology , myosin , endocrinology , biochemistry , gene
TPM1κ is an alternatively spliced isoform of the TPM1 gene whose specific role in cardiac development and disease is yet to be elucidated. Although mRNA studies have shown TPM1κ expression in axolotl heart and skeletal muscle, it has not been quantified. Also the presence of TPM1κ protein in axolotl heart and skeletal muscle has not been demonstrated. In this study, we quantified TPM1κ mRNA expression in axolotl heart and skeletal muscle. Using a newly developed TPM1κ specific antibody, we demonstrated the expression and incorporation of TPM1κ protein in myofibrils of axolotl heart and skeletal muscle. The results support the potential role of TPM1κ in myofibrillogenesis and sarcomeric function. J. Cell. Biochem. 110: 875–881, 2010. © 2010 Wiley‐Liss, Inc.