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CARM1 activates myogenin gene via PCAF in the early differentiation of TPA‐induced rhabdomyosarcoma‐derived cells
Author(s) -
Gao Xin,
Pan Weisong,
Dai Hui,
Zhang Ye,
Wu Ninghua,
Shen Yufei
Publication year - 2010
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22522
Subject(s) - pcaf , myogenin , gene knockdown , microbiology and biotechnology , biology , cancer research , cellular differentiation , gene , biochemistry , transcription factor
CARM1/PRMT4 is a member of the protein arginine methyltransferase (PRMT) family. CARM1 as a transcriptional coactivator plays an active role on mammalian genes. Here, we show that CARM1 can be recruited to the promoter of myogenin gene to enhance its transcriptional activation via PCAF at the early stage of TPA‐induced RD cell differentiation. By adding adenosine dialdehyde, AdOx, to inhibit the PRMT in RD cells, the TPA‐induced recruiting of p300, PCAF and the Brg1 at the myogenin promoter is abolished and myogenic differentiation is blocked. More specifically, the expression of PCAF and its nucleation are prohibited when CARM1 is knockdown by its specific siRNA. We suggest that the physical interaction of CARM1 and PCAF is likely pivotal for the activation of PCAF in the downstream of CARM1 pathway for inducing myogenin under TPA‐induced differentiation. The findings shed lights on novel therapeutic targets in the treatment of rhabdomyosarcoma patients. J. Cell. Biochem. 110: 162–170, 2010. © 2010 Wiley‐Liss, Inc.