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Increased integrin α 5 β 1 heterodimer formation and reduced c‐Jun expression are involved in integrin β 1 overexpression‐mediated cell growth arrest
Author(s) -
Fang Zhengyu,
Yao Wantong,
Fu Yi,
Wang LiYing,
Li Zengxia,
Yang Yong,
Shi Yinghong,
Qiu Shuangjian,
Fan Jia,
Zha Xiliang
Publication year - 2009
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22416
Subject(s) - integrin , integrin, beta 6 , cd49c , collagen receptor , microbiology and biotechnology , cell growth , downregulation and upregulation , protein subunit , cell culture , biology , extracellular matrix , chemistry , cell , biochemistry , gene , genetics
Integrins, heterodimers of α and β subunits, are a family of cell surface molecules mediating cell–cell and cell–extracellular matrix interaction. The largest subgroup is formed by the β 1 subunit containing integrins which consist of 12 members with different ligand‐binding properties. We previously reported that overexpressed integrin β 1 subunit in the hepatocellular carcinoma cell line SMMC‐7721 imposed a growth inhibitory effect through the upregulation of p21 cip1 and p27 kip1 . In this study, we confirmed the growth inhibitory effect of β 1 subunit overexpression in different cancer cell lines. The upregulated CDK inhibitors induced by β 1 integrin overexpression were essential for this integrin‐mediated growth arrest. Reduced c‐Jun level after integrin β 1 overexpression plays an important role in the transcriptional activation of p21 through the Sp1 sites. Solely overexpressed β 1 subunit could induce the expression of diverse α subunit in different cell lines, among which α 5 subunit was found to be correlated with integrin β 1 ‐mediated growth arrest. Relative lack of ECM–integrin interaction might be a reason for integrin β 1 overexpression‐mediated growth arrest. These results helped us understand more about the mechanisms that integrins regulate cell growth. J. Cell. Biochem. 109: 383–395, 2010. © 2009 Wiley‐Liss, Inc.