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Altered protein‐expressing profile in h PNAS4 ‐induced apoptosis in A549 human lung adenocarcinoma cells
Author(s) -
Gou LanTu,
Tong AiPing,
Yan Fei,
Yuan Zhu,
He Fei,
Wang Wei,
Zhou Yan,
Chen LiJuan,
Tang MingHai,
Yang JinLiang
Publication year - 2009
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22353
Subject(s) - apoptosis , western blot , annexin , a549 cell , microbiology and biotechnology , signal transduction , proteomics , biology , chemistry , inhibitor of apoptosis domain , blot , gene , biochemistry , programmed cell death , caspase
Human PNAS4 (h PNAS4 ) is a recently identified pro‐apoptosis gene, which is able to induce apoptosis in A549 human lung adenocarcinoma cells following its overexpression. In this work, we investigated the changes of protein profile in h PNAS4 ‐induced apoptosis in A549 cells through proteomic strategy consisting of two‐dimensional electrophoresis (2‐DE) coupled with MALDI‐Q‐TOF mass spectrometry. A total of 20 different proteins with more than 3.0‐fold change in expression, including 5 up‐regulated and 15 down‐regulated proteins were successfully identified by database search. The mRNA transcription levels of the different proteins were further examined by RT‐PCT. Functional analyses showed these different proteins are involved in diverse biological processes including metabolism, proteolysis, signal transduction, apoptosis, and redox regulation. Two essential apoptosis‐associated protein, annexin A1 and prothymosin alpha, were confirmed by Western blot and showed consistent changes with proteomic detection. Our data provide molecular evidence and possible associated pathway in h PNAS4 ‐induced apoptosis through proteomic strategy, which should be contributed to further investigation on biological function of h PNAS4 . J. Cell. Biochem. 108: 1211–1219, 2009. © 2009 Wiley‐Liss, Inc.