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Biochemical investigation of the effects of human platelet releasates on human articular chondrocytes
Author(s) -
Spreafico Adriano,
Chellini Federico,
Frediani Bruno,
Bernardini Giulia,
Niccolini Silvia,
Serchi Tommaso,
Collodel Giulia,
Paffetti Alessandro,
Fossombroni Vittorio,
Galeazzi Mauro,
Marcolongo Roberto,
Santucci Annalisa
Publication year - 2009
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22344
Subject(s) - platelet , chondrocyte , microbiology and biotechnology , platelet rich plasma , cell culture , cell , chemistry , articular cartilage , immunology , biology , in vitro , biochemistry , medicine , pathology , genetics , osteoarthritis , alternative medicine
The aim of the present study was to demonstrate the mitogenic and differentiating properties of platelet‐rich plasma releasates (PRPr) on human chondrocytes in mono‐ and three‐dimensional cultures. In order to assess if PRPr supplementation could maintain the chondrocyte phenotype or at least inhibit the cell de‐differentiation even after several days in culture, we performed a proteomic study on several cell cultures independently grown, for different periods of time, in culture medium with FCS, human serum (HS), and releasates obtained from PRP and platelet‐poor plasma (PPP). We found that PRP treatment actually induced in chondrocytes the expression of proteins (some of which novel) involved in differentiation. J. Cell. Biochem. 108: 1153–1165, 2009. © 2009 Wiley‐Liss, Inc.