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Three‐dimensional porous scaffolds at the crossroads of tissue engineering and cell‐based gene therapy
Author(s) -
Coutu Daniel L.,
Yousefi AzizehMitra,
Galipeau Jacques
Publication year - 2009
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22296
Subject(s) - genetic enhancement , tissue engineering , ex vivo , cell therapy , medicine , regenerative medicine , clinical trial , stem cell , bioinformatics , computational biology , in vivo , biology , microbiology and biotechnology , pathology , biomedical engineering , gene , genetics
In the last 20 years, more than 1,500 gene therapy clinical trials have been approved worldwide targeting a variety of indications, from inherited monogenic diseases to acquired conditions such as cancer, cardiovascular and infectious diseases. However, concerns about the safety and efficacy of gene therapy pharmaceuticals justify the development of alternative strategies to ensure the clinical translation of this still promising field. In particular, ex vivo gene therapy strategies using autologous adult stem cells coupled to three‐dimensional (3D) porous scaffolds show great promises in preclinical studies. Developments in the fields of biomaterial sciences and tissue engineering have already helped understanding how we can harness to regenerative potential of many cell types to create artificial tissues and organs and vastly improve the engraftment of ex vivo manipulated adult stem cells. In this article, we will review the current state of the art in tissue engineering by exploring the various types of clinically available biomaterials and the methods used to process them into complex 3D scaffolds. We will then review how these technologies are applied in cell‐based gene therapy and identify novel avenues of research that may benefit patients in the near future. J. Cell. Biochem. 108: 537–546, 2009. © 2009 Wiley‐Liss, Inc.