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Prostaglandin E 2 inhibits the proliferation of human gingival fibroblasts via the EP 2 receptor and Epac
Author(s) -
Weinberg Evgeny,
Zeldich Ella,
Weinreb Max M.,
Moses Ofer,
Nemcovsky Carlos,
Weinreb Miron
Publication year - 2009
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22242
Subject(s) - forskolin , ibmx , prostaglandin e , adenylate kinase , chemistry , endocrinology , medicine , mapk/erk pathway , cyclase , receptor , prostaglandin , fibroblast , cell growth , prostaglandin e2 , signal transduction , dna synthesis , microbiology and biotechnology , intracellular , protein kinase a , phosphorylation , biology , biochemistry , dna , in vitro
Elevated levels of prostaglandins such as PGE 2 in inflamed gingiva play a significant role in the tissue destruction caused by periodontitis, partly by targeting local fibroblasts. Only very few studies have shown that PGE 2 inhibits the proliferation of a gingival fibroblast (GF) cell line, and we expanded this research by using primary human GFs (hGFs) and looking into the mechanisms of the PGE 2 effect. GFs derived from healthy human gingiva were treated with PGE 2 and proliferation was assessed by measuring cell number and DNA synthesis and potential signaling pathways were investigated using selective activators or inhibitors. PGE 2 inhibited the proliferation of hGFs dose‐dependently. The effect was mimicked by forskolin (adenylate cyclase stimulator) and augmented by IBMX (a cAMP‐breakdown inhibitor), pointing to involvement of cAMP. Indeed, PGE 2 and forskolin induced cAMP generation in these cells. Using selective EP receptor agonists we found that the anti‐proliferative effect of PGE 2 is mediated via the EP 2 receptor (which is coupled to adenylate cyclase activation). We also found that the effect of PGE 2 involved activation of Epac (exchange protein directly activated by cAMP), an intracellular cAMP sensor, and not PKA. While serum increased the amount of phospho‐ERK in hGFs by ∼300%, PGE 2 decreased it by ∼50%. Finally, the PGE 2 effect does not require endogenous production of prostaglandins since it was not abrogated by two COX‐inhibitors. In conclusion, in human gingival fibroblasts PGE 2 activates the EP 2 —cAMP—Epac pathway, reducing ERK phosphorylation and inhibiting proliferation. This effect could hamper periodontal healing and provide further insights into the pathogenesis of inflammatory periodontal disease. J. Cell. Biochem. 108: 207–215, 2009. © 2009 Wiley‐Liss, Inc.