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Collection and culture of alveolar bone marrow multipotent mesenchymal stromal cells from older individuals
Author(s) -
Han Juan,
Okada Hironori,
Takai Hideki,
Nakayama Youhei,
Maeda Takahide,
Ogata Yorimasa
Publication year - 2009
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22224
Subject(s) - mesenchymal stem cell , bone marrow , klf4 , stromal cell , adipogenesis , microbiology and biotechnology , cell culture , chondrogenesis , biology , medicine , immunology , pathology , cancer research , gene , sox2 , genetics , embryonic stem cell
In this work, we examined the culture condition of alveolar bone marrow multipotent mesenchymal stromal cells (ABMMSCs), aiming to apply regenerative therapy to older periodontitis patients. To better understand the character of cultured cells from alveolar bone marrow, the expression profiles of well‐known genes and their responses to the induction of osteogenic, chondrogenic, or adipogenic differentiation were examined. Using αMEM‐based culture, ABMMSCs could be obtained from older individuals than in previous reports. Interestingly, ABMMSCs expressing Klf4 were able to differentiate into osteoblasts. The prediction of differentiation potential by Klf4 could be a useful guide for further improvement of the culture conditions required to culture ABMMSCs derived from older individuals. J. Cell. Biochem. 107: 1198–1204, 2009. © 2009 Wiley‐Liss, Inc.