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Study of the proinflammatory role of human differentiated omental adipocytes
Author(s) -
Bassols Judit,
Ortega Francisco J.,
MorenoNavarrete José M.,
Peral Belén,
Ricart Wifredo,
FernándezReal JoseManuel
Publication year - 2009
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22208
Subject(s) - proinflammatory cytokine , microbiology and biotechnology , biology , immunology , inflammation
Infiltration of monocyte‐derived macrophages into adipose tissue has been associated with tissue and systemic inflammation. It has been suggested that macrophage infiltration affects fat expansion through a paracrine action on adipocyte differentiation. Our working hypothesis is that factors released by monocytes/macrophages may also affect mature adipocyte biology. Human differentiated omental adipocytes were incubated with LPS and conditioned media obtained from human macrophage‐like cell line THP‐1, previously activated or not with LPS. We show that LPS greatly increased the secretion levels of pro‐inflammatory adipokines including IL‐6, IL‐8, GRO, and MCP‐1. Macrophage‐conditioned medium also upregulated IL‐6, IL‐8, GRO, and MCP‐1 mRNA expression and protein levels and led to the novo secretion of ICAM‐1, IL‐1β, IP‐10, MIP‐1α, MIP‐1β, VEGF, and TNFα. Human differentiated adipocytes treated by macrophage‐conditioned medium displayed marked reduction of adipocyte function as assessed by decreased phosphorylation levels of ERK1, ERK2, and p38α and reduced gene expression of lipogenic markers including PPAR‐γ and fatty acid synthase. These data show that macrophage‐secreted factors not only inhibit the formation of mature adipocytes but alter their function, suggesting that human differentiated omental adipocytes might also contribute to systemic chronic low‐grade inflammation associated with human obesity. J. Cell. Biochem. 107: 1107–1117, 2009. © 2009 Wiley‐Liss, Inc.