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Exogenous Nanog alleviates but is insufficient to reverse embryonic stem cells differentiation induced by PI3K signaling inhibition
Author(s) -
Liu Na,
Lu Min,
Feng Xiao Ming,
Ma Feng Xia,
Fang Zhi Hong,
Tian Xue Mei,
Ren Qian,
Zhang Lei,
Liu Bin,
Huang Ping Ping,
Liu Lin,
Han Zhong Chao
Publication year - 2009
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22082
Subject(s) - homeobox protein nanog , rex1 , embryonic stem cell , microbiology and biotechnology , ly294002 , nanog homeobox protein , pi3k/akt/mtor pathway , leukemia inhibitory factor , stem cell , cellular differentiation , biology , chemistry , signal transduction , induced pluripotent stem cell , biochemistry , gene
PI3K signaling pathway plays a significant role in embryonic stem cells (ES cells) self‐renewal. Overexpression of Nanog maintains mouse ES cells pluripotency independent of leukemia inhibitory factor (LIF). However, little is known about the effect of PI3K signaling pathway on ES cells with Nanog overexpression. Our experiments aimed to explore the relationship between PI3K signaling pathway and Nanog expression in ES cells. We observed the effect of LY294002, a specific inhibitor of PI3K pathway, on wild‐type J1 cells and Nanog overexpressing (Ex‐Nanog) J1 cells in the presence or absence of LIF. With LY294002 treatment, both of them lost their ES features even in the presence of LIF. But the differentiation induced by LY294002 on Ex‐Nanog J1 cells was slighter lower than that on wild‐type J1 cells. These results indicate that inhibition of PI3K pathway induces mouse ES cells differentiation. Exogenous Nanog sustains mouse ES cells pluripotency independent of LIF, and alleviates the differentiation induced by LY294002. But it is insufficient to totally reverse the differentiation. J. Cell. Biochem. 106: 1041–1047, 2009. © 2009 Wiley‐Liss, Inc.