Premium
IFI16 and NM23 bind to a common DNA fragment both in the P53 and the cMYC gene promoters
Author(s) -
Egistelli Lorenza,
Chichiarelli Silvia,
Gaucci Elisa,
Eufemi Margherita,
Schininà M. Eugenia,
Giorgi Alessandra,
Lascu Ioan,
Turano Carlo,
Giartosio Anna,
Cervoni Laura
Publication year - 2009
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.22053
Subject(s) - chromatin immunoprecipitation , promoter , nucleoside diphosphate kinase , microbiology and biotechnology , biology , dna , repressor , immunoprecipitation , gene , cell growth , gene expression , biochemistry
In the melanoma M14 cell line, we found that the antimetastatic protein NM23/nucleoside diphosphate kinase binds to the promoters of the oncogene cMYC and of P53, a gene often mutated in human cancer (Cervoni et al. [2006] J. Cell. Biochem. 98:421–428). In a further study, we find now that IFI16, a transcriptional repressor, in both promoters binds to the G‐rich fragment that also binds NM23/NDPK. These fragments possess non‐B DNA structures. Moreover, by sequential chromatin immunoprecipitation (re‐ChIP) we show that the two proteins (IFI16 and NM23/NDPK) are simultaneously bound in vivo to the same DNA fragments. Since P53 stimulates apoptosis and inhibits cellular growth, and cMYC promotes cell growth and, in several instances, also apoptosis, the presence of NM23 and IFI16 on the same DNA fragments suggests their common involvement in the reduced development of some tumors. J. Cell. Biochem. 106: 666–672, 2009. © 2009 Wiley‐Liss, Inc.