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Lipocalin‐2 is induced by interleukin‐1β in murine adipocytes in vitro
Author(s) -
Sommer Grit,
Weise Sebastian,
Kralisch Susan,
Lossner Ulrike,
Bluher Matthias,
Stumvoll Michael,
Fasshauer Mathias
Publication year - 2008
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21980
Subject(s) - lipocalin , downregulation and upregulation , adipocyte , insulin resistance , medicine , endocrinology , proinflammatory cytokine , interleukin 6 , secretion , cytokine , chemistry , interleukin , biology , insulin , inflammation , adipose tissue , biochemistry , gene
Lipocalin‐2 (Lcn2) has recently been isolated as an adipocyte‐secreted acute phase reactant that plays a role in insulin resistance, obesity, and atherosclerotic disease. In the current study, we determined regulation of Lcn2 by the proinflammatory and insulin resistance‐inducing cytokine interleukin (IL)‐1β in 3T3‐L1 and brown adipocytes by relative real‐time reverse transcription‐polymerase chain reaction. Interestingly, IL‐1β dramatically induced Lcn2 mRNA in both adipocyte models. Furthermore, Lcn2 protein secretion was dramatically upregulated in 3T3‐L1 adipocytes after 24 h of IL‐1β treatment. Experiments using pharmacological inhibitors indicated that IL‐1β‐induced Lcn2 expression is mediated via nuclear factor κB and janus kinase 2. Taken together, our results show an upregulation of Lcn2 by IL‐1β in fat cells implicating a potential role of this adipocyte‐secreted acute phase reactant in the development of insulin resistance, obesity, and associated disorders including cardiovascular disease. J. Cell. Biochem. 106: 103–108, 2009. © 2008 Wiley‐Liss, Inc.