Baicalein stimulates osteoblast differentiation via coordinating activation of MAP kinases and transcription factors
Author(s) -
Kim Jin Mi,
Lee SuUi,
Kim Young Sup,
Min Yong Ki,
Kim Seong Hwan
Publication year - 2008
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21760
Subject(s) - baicalein , osteoblast , kinase , microbiology and biotechnology , transcription factor , chemistry , runx2 , signal transduction , biology , biochemistry , pharmacology , gene , in vitro
Abstract The identification of anabolic agents that directly stimulate bone formation has recently attracted greater interest. Here, baicalein was identified as a natural compound that stimulates the differentiation of mouse osteoblastic MC3T3‐E1 subclone 4 cells. Baicalein induced the activation of NF‐κB in the initiation stage of osteoblast differentiation, and it activated the MAP kinase/NF‐κB signaling pathway and induced the expression of osteoblast differentiation markers in the early stage. In the late stage, baicalein stimulated the calcium deposition with the activation of MAP kinases and AP‐1 family members such as Fra‐1 and Fra‐2. Another transcription factor, NFATc1, was slightly induced by baicalein in the late stage. Thus, baicalein could stimulate the osteoblast differentiation via the activation of complexly coordinated signaling pathways that include MAP kinases and transcription factors such as NF‐κB, AP‐1, and NFATc1. J. Cell. Biochem. 104: 1906–1917, 2008. © 2008 Wiley‐Liss, Inc.
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