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Spatiotemporal dynamics of regulatory protein recruitment at DNA damage sites
Author(s) -
Mortusewicz Oliver,
Leonhardt Heinrich,
Cardoso M. Cristina
Publication year - 2008
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21751
Subject(s) - dna repair , dna damage , biology , epigenetics , dna , microbiology and biotechnology , computational biology , dna replication , nucleotide excision repair , g2 m dna damage checkpoint , cell , genetics , gene , cell cycle , cell cycle checkpoint
Mammalian cells are constantly threatened by multiple types of DNA lesions arising from various sources like irradiation, environmental agents, replication errors or by‐products of the normal cellular metabolism. If not readily detected and repaired these lesions can lead to cell death or to the transformation of cells giving rise to life‐threatening diseases like cancer. Multiple specialized repair pathways have evolved to preserve the genetic integrity of a cell. The increasing number of DNA damage sensors, checkpoint regulators, and repair factors identified in the numerous interconnected repair pathways raises the question of how DNA repair is coordinated. In the last decade, various methods have been developed that allow the induction of DNA lesions and subsequent real‐time analysis of repair factor assembly at DNA repair sites in living cells. This combination of biophysical and molecular cell biology methods has yielded interesting new insights into the order and kinetics of protein recruitment and identified regulatory sequences and selective loading platforms for the efficient restoration of the genetic and epigenetic integrity of mammalian cells. J. Cell. Biochem. 104: 1562–1569, 2008. © 2008 Wiley‐Liss, Inc.