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MMP‐14 mediated MMP‐9 expression is involved in TGF‐beta1‐induced keratinocyte migration
Author(s) -
Seomun Young,
Kim JongTak,
Joo ChounKi
Publication year - 2008
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21675
Subject(s) - hacat , matrix metalloproteinase , cell migration , keratinocyte , microbiology and biotechnology , cell , chemistry , transforming growth factor , cell growth , biology , in vitro , biochemistry
The importance of expression of matrix metalloproteinase (MMP) in keratinocyte migration is well established, but its role remains unclear. Here we investigated the function of MMP‐14 in TGF‐β1‐induced keratinocyte migration. TGF‐β1 stimulated cell migration and the expression of MMP‐2, ‐9 in HaCaT human keratinocyte cells. When we lowered MMP‐14 mRNA with siRNA, cell migration, and MMP‐9 expression decreased. Furthermore, the MMP‐14 siRNA also reduced activation of JNK in response to TGF‐β1, and a JNK‐specific inhibitor decreased both cell migration and MMP‐9 expression. Taken together, these results suggest that TGF‐β1‐induced HaCaT cell migration is mediated by MMP‐14, which regulates MMP‐9 expression via JNK signaling. J. Cell. Biochem. 104: 934–941, 2008. © 2008 Wiley‐Liss, Inc.