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Src‐Tyrosine kinases are major agents in mitochondrial tyrosine phosphorylation
Author(s) -
Tibaldi Elena,
Brunati Anna Maria,
Massimino Maria Lina,
Stringaro Annarita,
Colone Marisa,
Agostinelli Enzo,
Arancia Giuseppe,
Toninello Antonio
Publication year - 2008
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21670
Subject(s) - proto oncogene tyrosine protein kinase src , tyrosine phosphorylation , tyrosine protein kinase csk , fyn , phosphorylation , microbiology and biotechnology , receptor tyrosine kinase , sh2 domain , lyn , tyrosine , tyrosine kinase , mitochondrion , biology , protein tyrosine phosphatase , sh3 domain , signal transduction , protein phosphorylation , biochemistry , protein kinase a
Mitochondrial tyrosine phosphorylation is emerging as an important mechanism in regulating mitochondrial function. This article, aimed at identifying which kinases are the major agents in mitochondrial tyrosine phosphorylation, shows that this role should be attributed to Src family members. Indeed, various members of this family, for example, Fgr, Fyn, Lyn, c‐Src, are constitutively present in the internal structure of mitochondria as well as Csk, a key enzyme in the regulation of the activity of this family. By means of different approaches, biochemical fractioning, Western blotting and immunogold analysis “in situ” of phosphotyrosine signaling, evidence is reported on the existence of a signal transduction pathway from plasma membrane to mitochondria, resulting in increasing Src‐dependent mitochondrial tyrosine phosphorylation. The activation of Src kinases at mitochondrial level is associated with the proliferative status where several mitochondrial proteins are specifically tyrosine‐phosphorylated. J. Cell. Biochem. 104: 840–849, 2008. © 2008 Wiley‐Liss, Inc.