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Distinct osteoblastic differentiation potential of murine fetal liver and bone marrow stroma‐derived mesenchymal stem cells
Author(s) -
Fromigué Olivia,
Hamidouche Zahia,
Chateauvieux Sébastien,
Charbord Pierre,
Marie Pierre J.
Publication year - 2007
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21648
Subject(s) - osteopontin , bone morphogenetic protein 2 , bone marrow , osteoblast , stromal cell , mesenchymal stem cell , alkaline phosphatase , stem cell , haematopoiesis , osteocalcin , microbiology and biotechnology , chemistry , biology , endocrinology , medicine , immunology , cancer research , in vitro , biochemistry , enzyme
Bone marrow‐derived mesenchymal stem cells (MSC) are able to differentiate into osteoblasts under appropriate induction. Although MSC‐derived osteoblasts are part of the hematopoietic niche, the nature of the stromal component in fetal liver remains elusive. Here, we determined the in vitro osteoblastic differentiation potential of murine clonal fetal liver‐derived cells (AFT024, BFC012, 2012) in comparison with bone marrow‐derived cell lines (BMC9, BMC10). Bone morphogenetic protein‐2 (BMP2) increased alkaline phosphatase (ALP) activity, an early osteoblastic marker, in AFT024 and 2012 cells, whereas dexamethasone had little or no effect. BMP2, but not dexamethasone, increased ALP activity in BMC9 cells, and both inducers increased ALP activity in BMC10 cells. BMP2 increased ALP mRNA in AFT024, 2012 and BMC9 cells. By contrast, ALP was not detected in BMC10 and BFC012 cells. BMP2 and dexamethasone increased osteopontin and osteocalcin mRNA expression in 2012 cells. Furthermore, bone marrow‐derived cells showed extensive matrix mineralization, whereas fetal liver‐derived cell lines showed no or very limited matrix mineralization capacity. These results indicate that the osteoblast differentiation potential differs in bone marrow and fetal liver‐derived cell lines, which may be due to a distinct developmental program or different microenvironment in the two hematopoietic sites. J. Cell. Biochem. 104: 620–628, 2008. © 2007 Wiley‐Liss, Inc.