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The nuclear actin‐related protein Act3p/Arp4 influences yeast cell shape and bulk chromatin organization
Author(s) -
Georgieva Milena,
Harata Masahiko,
Miloshev George
Publication year - 2007
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21600
Subject(s) - chromatin , mutant , microbiology and biotechnology , biology , saccharomyces cerevisiae , yeast , gene , genetics
ACT3/ARP4 is an essential gene, coding for the actin‐related protein Act3p/Arp4 of Saccharomyces cerevisiae located within the nucleus. Act3p/Arp4 is a stoichiometric component of the NuA4, INO80, and SWR1 chromatin modulating complexes, and recruits these complexes onto chromatin for their proper chromatin functions. Mutated Act3p/Arp4 leads to impairment of the functions of these complexes and affects transcription of specific genes. Our results revealed significant disorder in the cell size and shape of act3/arp4 mutant cells, when grown at permissive temperature. act3/arp4 mutants have also demonstrated an increase in their nuclear diameters, thus suggesting that Act3p/Arp4 is a key regulator in the maintenance of cellular shape and nuclear organization. Furthermore, the use of Chromatin Yeast Comet Assay (ChYCA) for assessment of single‐cell bulk chromatin organization in act3/arp4 mutant cells allowed us to detect an elevated sensitivity toward nuclease action, denoting differences in higher‐order chromatin structure of the mutants. J. Cell. Biochem. 104: 59–67, 2008. © 2007 Wiley‐Liss, Inc.