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Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E‐cadherin
Author(s) -
Nagaoka Masato,
Ise Hirohiko,
Harada Ichiro,
Koshimizu Uichi,
Maruyama Atsushi,
Akaike Toshihiro
Publication year - 2007
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21406
Subject(s) - cadherin , microbiology and biotechnology , fibronectin , embryonic stem cell , cell adhesion , adhesion , biology , cell culture , cellular differentiation , p19 cell , cell adhesion molecule , cell , extracellular matrix , chemistry , biochemistry , adult stem cell , genetics , organic chemistry , gene
Rearrangement of cell–cell adhesion is a critical event in embryonic development and tissue formation. We investigated the regulatory function of E‐cadherin, a key adhesion protein, in the developmental process by using E‐cadherin/IgG Fc fusion protein as an adhesion matrix in cell culture. F9 embryonal carcinoma cells usually form colonies when cultured on gelatin or fibronectin matrices. However, F9 cells cultured on the E‐cadherin/IgG Fc fusion protein matrix formed a scattered distribution, with a different cytoskeletal organization and E‐cadherin‐rich protrusions that were regulated by Rac1 activity. The same scattering activity was observed in P19 embryonal carcinoma cells. In contrast, three types of differentiated cells, NMuMG mammary gland cells, MDCK kidney epithelial cells, and mouse primary isolated hepatocytes, did not show the scattering activity observed in F9 and P19 cells. These results suggest that migratory behavior on an E‐cadherin‐immobilized surface is only observed in embryonic cells, and that the regulatory mechanisms underlying E‐cadherin‐mediated cell adhesion vary with the state of differentiation. J. Cell. Biochem. 103: 296–310, 2008. © 2007 Wiley‐Liss, Inc.