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Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues
Author(s) -
Norris Russell A.,
Damon Brook,
Mironov Vladimir,
Kasyanov Vladimir,
Ramamurthi Anand,
MorenoRodriguez Ricardo,
Trusk Thomas,
Potts Jay D.,
Goodwin Richard L.,
Davis Jeff,
Hoffman Stanley,
Wen Xuejun,
Sugi Yukiko,
Kern Christine B.,
Mjaatvedt Corey H.,
Turner Debi K.,
Oka Toru,
Conway Simon J.,
Molkentin Jeffery D.,
Forgacs Gabor,
Markwald Roger R.
Publication year - 2007
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21224
Subject(s) - periostin , fibrillogenesis , chemistry , connective tissue , matricellular protein , microbiology and biotechnology , collagen, type i, alpha 1 , anatomy , biophysics , extracellular matrix , pathology , fibril , biology , biochemistry , medicine
Abstract Periostin is predominantly expressed in collagen‐rich fibrous connective tissues that are subjected to constant mechanical stresses including: heart valves, tendons, perichondrium, cornea, and the periodontal ligament (PDL). Based on these data we hypothesize that periostin can regulate collagen I fibrillogenesis and thereby affect the biomechanical properties of connective tissues. Immunoprecipitation and immunogold transmission electron microscopy experiments demonstrate that periostin is capable of directly interacting with collagen I. To analyze the potential role of periostin in collagen I fibrillogenesis, gene targeted mice were generated. Transmission electron microscopy and morphometric analyses demonstrated reduced collagen fibril diameters in skin dermis of periostin knockout mice, an indication of aberrant collagen I fibrillogenesis. In addition, differential scanning calorimetry (DSC) demonstrated a lower collagen denaturing temperature in periostin knockout mice, reflecting a reduced level of collagen cross‐linking. Functional biomechanical properties of periostin null skin specimens and atrioventricular (AV) valve explant experiments provided direct evidence of the role that periostin plays in regulating the viscoelastic properties of connective tissues. Collectively, these data demonstrate for the first time that periostin can regulate collagen I fibrillogenesis and thereby serves as an important mediator of the biomechanical properties of fibrous connective tissues. J. Cell. Biochem. 101: 695–711, 2007. © 2007 Wiley‐Liss, Inc.