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Phospholipase D1 activation through Src and Ras is involved in basic fibroblast growth factor‐induced neurite outgrowth of H19‐7 cells
Author(s) -
Oh DooYi,
Park ShinYoung,
Cho Ju Hwan,
Lee Ki Sung,
Min Do Sik,
Han JoongSoo
Publication year - 2006
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.21166
Subject(s) - neurite , basic fibroblast growth factor , phospholipase d , microbiology and biotechnology , proto oncogene tyrosine protein kinase src , signal transduction , growth cone , chemistry , growth factor , biology , receptor , in vitro , axon , biochemistry
Phospholipase D (PLD) is implicated in a variety of physiological processes that reveal it to be a member of the signal transducing phospholipases. We found that PLD1 is activated when basic fibroblast growth factor (bFGF) stimulates neurite outgrowth of an immortalized hippocampal cell line (H19‐7). Overexpression of PLD1 in H19‐7 cells dramatically elongated bFGF‐induced neurite outgrowth and increased PLD activity. Transfection of DN‐rPLD1 blocked bFGF‐induced PLD activation and completely inhibited neurite outgrowth induced by bFGF, suggesting that PLD1 activation is important in bFGF‐induced neurite outgrowth of H19‐7 cells. PLD activation and neurite outgrowth induced by bFGF was dependent on phospholipase C gamma (PLC‐γ) and Ca 2+ , but not protein kinase C (PKC). Furthermore, inhibition of Src and Ras partially blocked bFGF‐induced PLD activation and neurite outgrowth, respectively. Coinhibition of Src and Ras completely blocked bFGF‐induced PLD activation, suggesting that Src and Ras independently regulate PLD1 activation. Interestingly, bFGF‐induced PLD activation and neurite outgrowth did not require ERK1/2 activated by Ras. Taken together, this study demonstrates that bFGF activates PLD1 through PLC‐γ activation, which leads to neurite outgrowth in H19‐7 cells. Furthermore, our results show that PLD1 activation by bFGF is regulated by Src and Ras independently. J. Cell. Biochem. 101: 221–234, 2007. © 2006 Wiley‐Liss, Inc.